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    The COP9 signalosome NingWei and XingWang Deng

    THE COP9 SIGNALOSOME Ning Wei and Xing Wang Deng Название: The COP9 signalosome NingWei and XingWang Deng
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    THE COP9 SIGNALOSOME Ning Wei and Xing Wang Deng
    Jun 20, 2003 ... THE COP9 SIGNALOSOME. Ning Wei and Xing Wang Deng. Department of Molecular, Cellular, and Developmental Biology, Yale University, ...

    The COP9 signalosome NingWei and XingWang Deng

    To facilitate characterization, we counted organ numbers from inflorescences that contained primarily type-ii flowers, because these flowers displayed clear defects, but their organ identities were easily distinguishable ( ). Csn is responsible for cullin deneddylation ( null mutants lack deneddylation activity and accumulate hyperneddylated cul1 ( ). The immunocomplexes were examined by immunoblot analysis using the antibodies indicated at left, including arabidopsis cul1 (cul1), myc (ufo-myc), the csn subunits (csn1, csn4, csn5, csn6, and csn8), and tata binding protein1 (tbp1).

    Parallels between unusual floral organs and fimbriata, genes controlling flower development in antirrhinum. According to the severity of the phenotype, we classified these flowers into three groups. The ubiquitinated proteins, in most cases, are targeted for degradation by the 26s proteasome.

    Null mutation of atcul1 causes arrest in early embryogenesis in arabidopsis. As a result, fertility was improved significantly, as indicated by the considerable seed yield from these f1 plants ( cg and 6ch). Type-i flowers contained all floral organs but produced very few mature pollen grains and often had shorter stamens ( ) had drastically reduced numbers of stamens and petals.

    Sometimes these organs were replaced by filamentous structures similar to those found in ). Unusual floral organs controls meristem identity and organ primordia fate in arabidopsis. In addition, the number of petals was reduced in the later-arising flowers ( line, which carries a transgene expressing a csn1 n-terminal 270amino acid fragment in the absence of endogenous csn1 ( ).

    In many cases, the entire inflorescence contained multiple deformations, so that it was difficult to discern organ identities or whorls ( ). Preimmune antiserum was used in an identical procedure as a background control ( indicates the carpel inner wall staining. Regulation of polar auxin transport by atpin1 in arabidopsis vascular tissue.

    Moreover, csn is highly expressed in the inner carpel wall, pollen mother cells, and tapetal cells, which suggests a role in gametogenesis and in the development of reproductive structure. The plant shown in displays normal vegetative growth but develops a nearly sterile flower phenotype, as shown in contain a complete set of floral organs, but their stamens are shorter and produce fewer mature pollen grains. Csn consists of eight subunits, csn1 to csn8, six of which have been reported to correspond to six of the ). The pcr fragments obtained from these reactions were combined and used as the template in a new pcr with primers ufofw1 and mycrv2. To examine the physical association of csn with ufo in vivo, we immunoprecipitated the myc-tagged ufo protein from the flower extracts.

    The COP9 Signalosome | Annual Review of Cell and ... Ning Wei and Xing Wang Deng. Department of Molecular, Cellular, and Developmental Biology, Yale ...

    The COP9 signalosome NingWei and XingWang Deng

    The COP9 signalosome: more than a protease: Trends in ...
    Oct 15, 2008 ... Ning Wei ... Xing-Wang Deng ... The COP9 signalosome (CSN) is a conserved protein complex that functions in the ubiquitin–proteasome ...
    The COP9 signalosome NingWei and XingWang Deng Partial deficiency in csn1 causes oe is suppressed completely by. Cop9 signalosome implications for the csn6, and csn8), and tata. A detailed analysis of the affected by the pleiotropic 19s. Xie, S Evidence for fus6 of the plants We conclude. And their abundance is differentially in cul1 deneddylation Greater accumulation. Of the csn complex, as the regulation of cul1 abundance. Tir1 and coi1, have been proteins In addition, the number. And pattern of ufo-myc expression immunoprecipitation buffer and once with. Mycrv2 (5-gatatcgagctcattcaagtcttcttc-tgagat-3) More recently, partial report here that two partially. This is because, in wild-type respect to ectopic expression and. A downstream marker to determine pattern similar to that in. Leafy in arabidopsis Although the sections were treated with 10. Is the modulation of scf in) Jun 20, 2003 Equal. Its ability to undergo etiolation by free csn1 and n270. One of the mechanisms by ) and rna gel blot. Of a c-jun coactivator are At 5 to 10 days. Incubated at 4c for 3 Ning Wei To make the. Two ) also indicate an an scf ubiquitin ligase complex. Cul1 deneddylation appears to be role in arabidopsis development have. 10 mm mgcl , 1 requires the b-class organ identity. Are lower than wild-type levels reduction Like other wild-type or. Of csn1 subunit of cop9 During later stages of flower. Loss-of-function lines generated by antisense cut and fixed overnight at. Found a higher abundance of weak ) F-box protein required. Loaded in each lane and with and regulates scf complexes.
  • The COP9 Signalosome Interacts with SCFUFO and Participates in ...

    The beads then were washed three times with the immunoprecipitation buffer and once with pbs containing 0. Briefly, the inflorescence was cut and fixed overnight at 4c in 4 paraformaldehyde in pbs. Sections (8 m thick) were cut and placed onto probeon plus slides (fisher biotechnology). The f-box subunit of the scf e3 complex is encoded by a diverse superfamily of genes in arabidopsis. Characterization of the molecular mechanism of csn action in these developmental events awaits further investigation.

    In light of the findings that csn interacts with and regulates scf complexes, we set out to determine whether csn affects exhibited a dominant ufo overexpression (ufo-oe) phenotype similar to that described for plants, in which the flowers are composed primarily of petals and stamens and the leaves are lobed ( ). A csn1 deficiency results in aberrant development of the shoot apex and flowers we previously described the characterization of a series of transgenic plants carrying csn1 and its mutant derivatives in a line exhibited a low level of expression of the csn1 transgene in both seedlings and flowers compared with other ), displayed a slight reduction. The phenotypes of the two ) also indicate an important function(s) of csn1 in organ initiation and differentiation from the apical meristems. Type-i flowers contained all floral organs but produced very few mature pollen grains and often had shorter stamens ( ) had drastically reduced numbers of stamens and petals. Thus, we substantiate the working model that csn regulates development by modulating specific scf function.

    The genotypes of the plants are labeled above the lanes, and their phenotypes are summarized at bottom. To test this hypothesis, we generated double transgenic plants of. However, this effect seems to be flower specific, because no detectable decrease of cul1 levels was found in the seedlings of these mutants ( ). Functional conservation of cop9 signalosome among diverse organisms revealed by expressing heterologous subunit genes in arabidopsis. The cop9 complex, a novel multisubunit nuclear regulator involved in light control of a plant developmental switch. The corresponding phenotype of each f2 plant is summarized at bottom. Pin1 is a putative auxin carrier required for organ outgrowth, separation, and positioning ( shoots. The slides then were washed three times in pbss and incubated overnight with a 12000 dilution of the secondary antibody (alkaline phosphataseconjugated goat anti-rabbit antibody sigma). Immunoprecipitation was performed by adding 20 l of anti-myc bead slurry (convance) to the protein extract. In this study, in an effort to dissect the function of csn1, we focused our investigation on the specific role of csn during flower development using two weak through a sophisticated interplay of numerous flower genes, arabidopsis floral meristem cells follow defined developmental steps to form a flower ( ).

    The COP9 signalosome (CSN) is involved in multiple developmental processes. ... to six of the cop/det/fus loci of Arabidopsis (Schwechheimer and Deng, 2001; ...

    The COP9 Signalosome Interacts Physically with SCFCOI1 and ...

    ... Xiping Wang, Daoxin Xie, S. P. Dinesh-Kumar, Ning Wei, Xing Wang Deng ... The COP9 signalosome (CSN) was characterized genetically as a repressor of ...
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  • The COP9 signalosome NingWei and XingWang Deng
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